human duo set elisa kits (R&D Systems)
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
94
Structured Review
R&D Systems
human duo set elisa kits
Human Duo Set Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+kit+duoset/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pmc13122707-57-22-27
Average 94 stars, based on 47 article reviews
Human Duo Set Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+kit+duoset/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pmc13122707-57-22-27
Average 94 stars, based on 47 article reviews
human duo set elisa kits - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC"
Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC
Journal: Redox Biology
doi: 10.1016/j.redox.2026.104172
Figure Legend Snippet: Characterization of IL-1α overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, ELISA and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
Techniques Used: Over Expression, Construct, Gene Expression, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Control
Figure Legend Snippet: IL-1α overexpression alters expression of IL-1 pathway genes. Gene expression of IL1B (A), IL6 (B), IL8 (C), IL1RN (D), IL1RAP (E) and IL1R1 (F) in the three IL1A constructs – Full-Length (FL), N-terminal (NT) and C-terminal (CT) in Cal27 IL-1αoverexpressing cells were analyzed by RT-PCR using GAPDH as an endogenous control. Protein secretion of IL-1β (G), IL-6 (H), IL-8 (I), and IL1RA (J) in cell culture supernatants was quantified by ELISA with protein concentrations normalized to cell numbers. Whole cell lysates were analyzed for IL-1R1 expression by Western blot using GAPDH as a loading control (K). Bars represent mean ± SEM from n = 3 independent experiments.∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.
Techniques Used: Over Expression, Expressing, Gene Expression, Construct, Reverse Transcription Polymerase Chain Reaction, Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot
Related Articles
Incubation:Article Title: Protective immunity induced by DNA-library immunization against an intracellular bacterial infection. Article Snippet: DNA-based immunization has shown to be a viable alternative approach to induce protective immunity against Brucella abortus infection.. However, the use of a unique gene may not be sufficient to induce full protection.. Therefore, a new strategy based on library immunization has been described to improve the level of protection against different pathogens and to identify new protective genes. Article Title: Synergy and oxygen adaptation for development of next-generation probiotics Article Snippet: Caco-2 from the European Collection of Cell Cultures (batch 18H036, Merck) were cultured in supplemented Dulbecco’s modified Eagle’s medium (DMEM) (PAA Laboratories) at 37 °C in a 5% CO2 incubator. .. Cells were incubated with different F. prausnitzii supernatant fractions cultured in LYBHI (1:25 and 1:10 in DMEM medium) and stimulated with (4 ng ml −1 IL-1β) for 6 h. IL-8 levels were determined in duplicate in cell supernatants using Enzyme-linked Immunosorbent Assay:Article Title: Protective immunity induced by DNA-library immunization against an intracellular bacterial infection. Article Snippet: DNA-based immunization has shown to be a viable alternative approach to induce protective immunity against Brucella abortus infection.. However, the use of a unique gene may not be sufficient to induce full protection.. Therefore, a new strategy based on library immunization has been described to improve the level of protection against different pathogens and to identify new protective genes. Article Title: Suppressing Endoplasmic Reticulum Stress Alleviates LPS-Induced Acute Lung Injury via Inhibiting Inflammation and Ferroptosis. Article Snippet: Acute lung injury (ALI) is a life-threatening clinical disorder with high mortality rate.. Ferroptosis is a new type of programmed cell death with lipid peroxidation and iron ion overloading as the main characteristics.. Endoplasmic reticulum (ER) stress and ferroptosis play pivotal roles in the pathogenesis of ALI. Article Title: Synergy and oxygen adaptation for development of next-generation probiotics Article Snippet: Caco-2 from the European Collection of Cell Cultures (batch 18H036, Merck) were cultured in supplemented Dulbecco’s modified Eagle’s medium (DMEM) (PAA Laboratories) at 37 °C in a 5% CO2 incubator. .. Cells were incubated with different F. prausnitzii supernatant fractions cultured in LYBHI (1:25 and 1:10 in DMEM medium) and stimulated with (4 ng ml −1 IL-1β) for 6 h. IL-8 levels were determined in duplicate in cell supernatants using Article Title: DNA Vaccine Using Mycobacterium bovis Ag85B Antigen Induces Partial Protection against Experimental Infection in BALB/c Mice Article Snippet: .. After 72 h at 37°C in air with 5% CO 2 , splenocyte culture supernatants were tested for the presence of cytokines with the Article Title: Identification of an anti-inflammatory protein from Faecalibacterium prausnitzii , a commensal bacterium deficient in Crohn's disease Article Snippet: Peptide syntheses were carried out on a 0.1-mmol scale using an ABI Model 431A peptide synthesizer (Applied BioSystems), starting from the appropriate Wang Tentagel resin, with 10 equiv. of the protected Fmoc-amino acid and HBTU/DIEA for the activation. .. The IL-8 level was determined in duplicate in cell supernatants using Article Title: DNA Vaccine Using Mycobacterium bovis Ag85B Antigen Induces Partial Protection against Experimental Infection in BALB/c Mice Article Snippet: .. After 72 h at 37°C in air with 5% CO2, splenocyte culture supernatants were tested for the presence of cytokines with the Article Title: Curcumin-loaded nanoemulsion for acute lung injury treatment via nebulization: Formulation, optimization and in vivo studies Article Snippet: .. Cytokine levels for TNF-α and IL-1β were measured in the BALF supernatant by the ELISA kit according to the manufacturer’s instructions ( Cell Culture:Article Title: Synergy and oxygen adaptation for development of next-generation probiotics Article Snippet: Caco-2 from the European Collection of Cell Cultures (batch 18H036, Merck) were cultured in supplemented Dulbecco’s modified Eagle’s medium (DMEM) (PAA Laboratories) at 37 °C in a 5% CO2 incubator. .. Cells were incubated with different F. prausnitzii supernatant fractions cultured in LYBHI (1:25 and 1:10 in DMEM medium) and stimulated with (4 ng ml −1 IL-1β) for 6 h. IL-8 levels were determined in duplicate in cell supernatants using |